Simple and rapid method for analysis of urinary vancomycin using solid phase extraction and fluorescence spectroscopy

Abstract Vancomycin (VCM) is an antimicrobial that recommended for therapeutic drug monitoring (TDM) maintaining the efficacy and safety of treatment. The trough has been used to guide VCM dosing regimens. However, newer guidelines recommend use area under curve/minimum inhibitory concentration (AUC/MIC)-guided vancomycin dosing, there a need easier more frequent measurements concentrations. Therefore, in this study, we developed simple rapid analytical method measuring urinary by combining solid-phase extraction fluorescence analysis. Urine samples are less invasive than blood samples. In addition range VCM, was also able measure 0.01–1 mg/mL, which VCM. accuracy 10, 20, 30 μg/mL solutions were between 93.18 109.76%. relative standard deviation (RSD) intra-day inter-day analysis 6.25% 6.28%, respectively. Since does not large equipment, it expected be better suited clinical use.